Journal: The European Journal of Neuroscience
Article Title: Ventral Hippocampus Modulates Prefrontal Control of Background Contextual Fear After Cued Extinction
doi: 10.1111/ejn.70287
Figure Lengend Snippet: vHPC‐PL pathway stimulation effects on contextual fear expression. (a) Top, experimental design of the compound ABCA fear renewal protocol. Optogenetic stimulation of the vHPC‐PL pathway took place during the baseline pre‐CS presentation periods in both renewal test sessions. All sessions took place on different days. Bottom, comparison of the average time spent freezing during the different learning stages of the protocol for the stimulated animals (ChR2, n = 7) and controls ( n = 7). Whiskers depict ±SEM, the shaded blue rectangle indicates when laser was ON. [n.s.: no significant difference between groups; *: t (12) = 2.51, p = 0.027; unpaired t ‐test, see Section and Data ]. (b) Top, experimental design of the contextual fear conditioning and recall test protocol. Bottom, comparison of percentage of time spent freezing during the baseline and the inter‐trial intervals (ITI) of the contextual fear conditioning session (left) and during the 6 min of the contextual fear recall test (right) for the laser stimulated group ( n = 9) and control animals ( n = 19). Whiskers depict ±SEM, the shaded blue rectangle indicates when laser was ON. [No significant difference at any time point; unpaired t ‐test, see Section and Data ]. (c) Top, experimental design of the ABA fear renewal protocol. Below, comparison of the average time spent freezing during the different learning stages of the protocol for the stimulated animals (ChR2, n = 10) and controls ( n = 18). Same format as panels (a) and (b). CS 1–4 = average freezing over the first four CS presentations. [*: t (24) = 2.80, p = 0.010; unpaired t ‐test, see Section and Data ]
Article Snippet: All training and test sessions were conducted in a standard conditioning chamber kept inside a sound‐attenuating cubicle (VCF‐007, Med Associates, USA).
Techniques: Expressing, Comparison, Control
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![vHPC‐PL pathway stimulation effects on contextual fear expression. (a) Top, experimental design of the compound ABCA fear renewal protocol. Optogenetic stimulation of the vHPC‐PL pathway took place during the baseline pre‐CS presentation periods in both renewal test sessions. All sessions took place on different days. Bottom, comparison of the average time spent freezing during the different learning stages of the protocol for the stimulated animals (ChR2, n = 7) and controls ( n = 7). Whiskers depict ±SEM, the shaded blue rectangle indicates when laser was ON. [n.s.: no significant difference between groups; *: t (12) = 2.51, p = 0.027; unpaired t ‐test, see Section and Data ]. (b) Top, experimental design of the contextual fear <t>conditioning</t> and recall test protocol. Bottom, comparison of percentage of time spent freezing during the baseline and the inter‐trial intervals (ITI) of the contextual fear conditioning session (left) and during the 6 min of the contextual fear recall test (right) for the laser stimulated group ( n = 9) and control animals ( n = 19). Whiskers depict ±SEM, the shaded blue rectangle indicates when laser was ON. [No significant difference at any time point; unpaired t ‐test, see Section and Data ]. (c) Top, experimental design of the ABA fear renewal protocol. Below, comparison of the average time spent freezing during the different learning stages of the protocol for the stimulated animals (ChR2, n = 10) and controls ( n = 18). Same format as panels (a) and (b). CS 1–4 = average freezing over the first four CS presentations. [*: t (24) = 2.80, p = 0.010; unpaired t ‐test, see Section and Data ]](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8305/pmc12548305/pmc12548305__EJN-62-0-g001.jpg)